2.7.7.67 | purified recombinant enzyme in the CTP- and Mg2+-bound state, mixing of 25 mg/ml protein in 10 mM Tris-HCl, pH 8.0, containing 100 mM NaCl, 0.02% dodecyl-beta-D-maltopyranoside, and 10% glycerol with 200 mM NaCl, 20% w/v PEG 400, and 100 mM Tris-HCl, pH 8.0, at 20°C, 3 weeks, X-ray diffraction structure determination and analysis at 2.4 A resolution, structure modeling |