EC Number   |
Reference |
|---|
    2.7.7.3 | - |
663331 |
| 2.7.7.3 | apo form and in complex with ATP, counter-diffusion method, using 40 mM cacodylate buffer pH 5.5 containing 10 mM MgCl2, 0.15 mM NaCl, 20 mM cobalt hexamine and 15% (w/v) 2-methyl-2,4-pentanediol as precipitant solution |
721166 |
| 2.7.7.3 | at pH 5.0, 100 mM sodium acetate, pH 5.0, 1.1 M ammonium sulfate, 200 mM NaCl |
674344 |
| 2.7.7.3 | co-crystallization with CoA, space group: I23, with a dimer in the asymmetric unit, a solvent content of 0.57 and a volume-to-protein mass ratio of 288 A3 Da-1 |
643185 |
| 2.7.7.3 | co-crystallization with inhibitor PTX040334 in 5% DMSO, 22-32% polyethylene glycol 8000, 200 mM ammonium sulfate in 100 mM cacodylate buffer, pH 6-6.5 at 21°C |
643184 |
| 2.7.7.3 | co-crystallization with pantetheine 4'-phosphate or ATP, space group: I23, with a dimer in the asymmetric unit, a solvent content of 57% and a volume-to-protein mass ratio of 288 A3 Da-1 |
643186 |
| 2.7.7.3 | enzyme crystals are grown in microgravity by the capillary counter-diffusion method through a gel layer, mixing of 10 mg/ml protein in 10 mM HEPES, pH 8.0, 0.15 M NaCl, and 14 mM ATP, with reservoir solution containing 0.1 M NaAc, pH 5.0, 10 mM MgCl2, 5 mM HEPES, pH 8.0, 0.075 M NaCl, 14 mM ATP, and 1.1 M ammonium sulfate, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement method |
738084 |
| 2.7.7.3 | enzyme from Staphylococcus aureus subsp. aureus MW2 in complex with inhibitors, vapour diffusion method, mixing of 0.0025 ml of protein solution with 0.0025 ml of reservoir solution containing 14% to 19% PEG 3350, 200 mM ammonium sulfate, and 0.1 M propionic acid cacodylate Bis-Tris propane buffer, pH 7.5, 20°C, 5-7 days, X-ray diffraction structure determination and analysis at 1.72-2.38 A resolution, molecular replacement and modelling of inhibitor binding |
737460 |
| 2.7.7.3 | enzyme in complex with pantetheine 4'-phosphate and adenosine-5'-[(alpha,beta)-methyleno]triphosphate, sitting drop vapor diffusion method, using 38% (v/v) polyethylene glycol 200 and 0.1 M HEPES (pH 7.2), at 21°C |
722971 |
| 2.7.7.3 | hanging drop vapor diffusion method |
660591 |
