EC Number   |
|---|
    2.7.7.2 | - |
| 2.7.7.2 | 3D structural model for based on the structure of Thermotoga maritima FADS |
| 2.7.7.2 | crystal structure predicts a dimer of trimers organization |
| 2.7.7.2 | hanging-drop vapor-diffusion method, 277 K |
| 2.7.7.2 | homology modeling of structure |
| 2.7.7.2 | in complex with FAD, hanging drop vapor diffusion method |
| 2.7.7.2 | mutant enzyme E268D, hanging drop vapor diffusion method |
| 2.7.7.2 | purified recombinant DELTA(1-182)CaFADS module in binary complex with ADP-Ca2+ and in ternary complex with FMN-ADP-Mg2+, mixing of 0.002 ml of 7.5-10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM MgCl2, 1mM FMN and/or 1 mM ADP, with 0.002 ml of reservoir solution containing 10-14% PEG 8000, 20% glycerol, 0.1 M MES-NaOH pH 6.5, 200 mM CaCl2 for the binary complex, or with 0.002 ml of reservoir solution containing 26-30% PEG 4000, 200 mM Li2SO4, 100 mM sodium acetate, pH 5.0, as well as 0.002 ml of 1 M NaI solution, for the ternary complex, X-ray diffraction structure determination and analysis at 1.65-2.15 A resolution, modelling |
| 2.7.7.2 | purified recombinant enzyme mutant R66A and R66E, mixing of equal volumes of 10 mg/ml protein in 20mMTris/HCl, pH 8.0, and 1 mM DTT, with reservoir solution containing 1.5 M Li2SO4, 0.1 M HEPES/NaOH, pH 7.5, X-ray diffraction structure determination and analysis, molecular replacment and modelling using the native CaFADS structure, PDB ID 2X0K, as search model |
