    2.7.1.45 | 2 enzyme crystal forms, X-ray diffraction structure determination and analysis at 2.3 and 3.2 A resolution, respectively, molecular replacement, enzyme in complex with ATP, with 2-dehydro-3-deoxy-D-gluconate and ATP analogue adenosine 5'-(beta,gamma-imino)triphosphate, i.e. AMP-PNP, or with 2-dehydro-3-deoxy-D-gluconate and ATP, X-ray diffraction structure determination and analysis at 2.1 A resolution |
659714 |
| 2.7.1.45 | purified recombinant enzyme, free enzyme or in complex with ATP and 2-dehydro-3-deoxy-D-gluconate, method 1: automated microbatch method, 500 nl 23 mg/ml protein mixed with 500 nl screen solution containing 44% v/v methylpentanediol, 10% v/v dioxane, 0.1 M Na HEPES, pH 7.7, and covered with 0.015 ml silicone and parraffin oil mixture, 18°C, crystals grow to final dimensions within 1 month after appearance, method 2: sitting drop vapour diffusion method at 25°C, 0.001 ml 10 mg/ml protein solution mixed with equal volume of reservoir solution and equilibrated against 0.1 ml reservoir solution containing 28% v/v methylpentanediol, 10 mM CaCl2, 0.1 M trisodium citrate buffer, pH 5.6, or for cocrystallization of enzyme with ligands containing 0.35-0.45 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.1-3.2 A resolution |
657469 |
