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EC Number Crystallization (Commentary)
Show all pathways known for 2.7.1.22Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.22hanging drop vapor diffusion, 10 mg/ml protein in 100 mM Hepes, pH 7.2, 300 mM NaCl, 1 mM dithiothreitol are incubated with 3 mM NAD and 3 mM ATP and mixed with an equal volume of the reservoir solution consisting of 100 mM MES, pH 6.0, 1 M ammonium sulfate, crystal structure of enzyme complexed with NAD
Show all pathways known for 2.7.1.22Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.22sitting-drop vapor-diffusion method at 22°C, crystal structures of NRK1 in a binary complex with the reaction product nicotinamide mononucleotide at 1.5 A resolution and in a ternary complex with ADP and tiazofurin at 2.7 A resolution. The active site is located in a groove between the central parallel beta sheet core and the LID and NMP-binding domains
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