EC Number   |
|---|
   2.7.1.180 | analysis of X-ray crystal structure of the Vibrio cholerae Nqr complex and structures of several purified components of the complex, PDB IDs 4U9S and 4P6V |
| 2.7.1.180 | purified Ftp_Ec mutant variant Y60N complexed with ADP, hanging drop vapor diffusion method, mixing of 0.004 ml of 20 mg/ml protein in Tris, pH 7.5, and 20 mM NaCl, with 0.04 ml of reservoir solution containing 0.2 M NH4NO3, and 20% w/v PEG 3350, and 0.001 ml of 50 mM MgCl2, and 0.001 ml of 50 mM ADP, 20°C, X-ray diffraction structure determination and analysis at 1.85 A resolution, molecular replacement and modelling |
| 2.7.1.180 | purified recombinant His-tagged wild-type ApbE and H257G mutant, sitting drop vapor diffusion technique, mixing of protein in 10 mM HEPES, 150 mM NaCl, 0.5 mM FAD, and 5 mM MgCl2, pH 8.0, with crystallization solution containing 0.1 M sodium acetate, 25% PEG 4000, and 8% isopropyl alcohol for the wild-type enzyme, and 0.1 M sodium acetate, 22% PEG 4000, and 0.1 M HEPES buffer, pH 7.5, for the mutant H257G enzyme, 16°C, X-ray diffraction structure determination and analysis at 1.61 A and 1.92 A resolution, respectively |
| 2.7.1.180 | purified recombinant wild-type enzyme Ftp_Ec, mutant E169K, and mutant Y60N bound to ADP, hanging drop vapor diffusion method, mixing of 0.004 ml of 20 mg/ml protein in 20 mM Tris, pH 7.5, and 20 mM NaCl, with 0.004 ml of reservoir solution containing 0.2 M NH4NO3 and 20% w/v PEG 3350 for the wild-type, and 25% w/v PEG 1500, 0.1 M MIB (malonate, imidazole and boric acid), pH 5.0 for the mutant, 20°C, X-ray diffraction structure determination and analysis at 1.75-1.88 A resolution |
