2.6.1.92 | crystal structure of the native protein, its complexes with pyridoxal 5'-phosphate alone and in combination with UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine. A crystal of apo-PseC is obtained in 2 days at 20°C from a hanging drop by mixing 0.0015 ml of protein in buffer with 0.0015 ml of reservoir solution containing 0.2 M ammonium acetate, 0.1 M trisodium citrate, pH 5.6, 30% polyethylene glycol 4000. The crystals belong to space group P2(1)2(1)2 with unit cell dimensions a = 87.7, b = 155.4, and c = 71.5 A and Z = 4 |
715485 |