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Results 1 - 6 of 6
EC Number Crystallization (Commentary)
Show all pathways known for 2.6.1.57Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.57ARO9 by hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 2.6 A resolution
Show all pathways known for 2.6.1.57Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.57crystal structure of unliganded pdAroAT, pdAroAT in a complex with maleate and 3-phenylpropionate at 2.33 A, 2.5 A and 2.3 A resolution
Show all pathways known for 2.6.1.57Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.57hanging drop vapor diffusion, an equi-volume of 3 M 1,6-hexanediol solution at pH 7.5, 100 mM HEPES buffer, containing 10 mM MgCl2 is added to a protein solution containing 1.6% AeATPh and 0.02 mM pyridoxal 5'-phosphate, a droplet of the solution is equilibrated with 1 ml of 3 M 1,6-hexane-di-ol solution, crystals are grown at room temperature for 1 week, crystal structure of the native enzyme 2.1 a resolution, heavy atom derivatives diffract to 3.0 A resolution
Show all pathways known for 2.6.1.57Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.57hanging-drop vapor diffusion method at 20°C using 3 M 1,6-hexanediol, 100 mM HEPES (pH 7.5), and 10 mM MgCl2 as the precipitant solution. The crystal structure of the ligand-free enzyme is analyzed at a resolution of 1.8 A
Show all pathways known for 2.6.1.57Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.57purified recombinant enzyme, hanging drop vapor diffusion method, mixing of 0.001 ml of 18.0 mg/ml protein in 25 mM Tris-HCl, pH 7.0, with 0.001 ml of reservoir solution containing 0.2 M potassium chloride, pH 7.0, and 20% w/v PEG 3550, and equilibration against 0.02 ml reservoir, X-ray diffraction structrue determination and analysis at 2.2 A resolution, molecular replacement method using the crystal structure of AroAT from Pyrococcus horikoshii strain OT3 (PDB ID 1dju) as initial search model, modeling
Show all pathways known for 2.6.1.57Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.57tertiary structures of pdAroAT complexed with nine kind of inhibitors: 3-indolebutyric acid, 4-phenylbutyric acid, 5-phenylvaleric acid, 4-(2-thienyl)butyric acid, cyclohexanepropionic acid, 4-amminohydrocinnamic acid, 3-(p-tolyl)-propionic acid and 3-(3,4-dimethoxyphenyl)propionic acid, crystals of the maleate complex of pdAroAT are made by the micro-seeding method using 24% poly(ethylene)glycol 4000, 200 mM sodium maleate, pH 5.7 and 5 mM MgCl2 as precipitating buffer, cocrystallized inhibitor maleate is replaced by soaking the crystals in 24% poly(ethylene)glycol 4000, 100 mM sodium citrate, pH 5.7 containing 100 mM inhibitor for 10 h
Results 1 - 6 of 6