EC Number   |
Reference |
|---|
    2.5.1.7 | at 19°C, from 10 mM MES, pH 6.4, 10% (w/v) polyethylene glycol 20000, in the presence of 5 mM UDP-N-acetyl-D-glucosamine and 5 mM phosphoenolpyruvate |
674498 |
| 2.5.1.7 | crystallization of substrate-free enzyme, two-domain structure with an unusual fold, inside out alpha/beta barrel, which is built up from the 6fold repetititon of one folding unit, structure analysis |
637600 |
| 2.5.1.7 | crystallization of the enzyme complexed with UDP-N-acetylglucosamine and fosfomycin, two-domain structure with the active site located between them, structure and substrate binding analysis |
637599 |
| 2.5.1.7 | in complex with inhibitor cnicin and substrate UDP-N-acetylglucosamine, at 2.0 A resolution. The enzyme catalyzes the formation of a covalent adduct between cnicin and UDP-N-acetylglucosamine via an anti-Michael 1,3-addition of UDP-N-acetylglucosamine to an alpha,beta-unsaturated carbonyl function in cnicin thus forming a noncovalent suicide inhibitor |
688290 |
| 2.5.1.7 | in complex with inhibitor T6361 |
659457 |
| 2.5.1.7 | in complex with substrates, hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 5.5), 0.2 M NH4OAc, 25% (w/v) PEG 3350 |
722746 |
| 2.5.1.7 | in complex with UDP-N-acetylglucosamine and fosfomycin, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M bis-Tris pH 6.5 |
721232 |
| 2.5.1.7 | in complex with UDP-N-acetylglucosamine and fosfomycin, to 2.3 A resolution. The active-site loop can adopt one of the three major conformations: a fully open conformation, a half-open conformation, and a closed conformation. Fosfomycin can bind without inducing a large change in the half-open conformation of the binary complex with UDP-N-acetylglucosamine |
689975 |
| 2.5.1.7 | in the apo-form, as well as in a complex with UDP-N-acetylglucosamine and fosfomycin using ammonium sulfate as the precipitant, hanging drop vapour diffusion method |
675932 |
| 2.5.1.7 | MurA is crystallized in the presence of sodium phosphite and UDP-N-acetylmuramic acid using the hanging drop vapor diffusion method, the MurA cocrystal structure with UDP-N-acetylmuramic acid and phosphite reveals a new staged MurA conformation in which the Arg397 side chain tracked phosphite out of the catalytic site |
702267 |
