2.5.1.66 | crystal structures of tetrameric CEAS-thiamin diphosphate in complex with the substrate analogues 5-guanidinovaleric acid and tartrate, crystals of 2 morphologies are grown by the hanging-drop vapour diffusion technique using 1.6 M ammonium sulfate as precipitant, in the presence of 0.1 M HEPES, pH 7.4, and a 3fold molar excess of thiamin diphosphate |