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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.66CEAS complexed with thiamine diphosphate and Mg2+, selenomethionine-substituted CEAS, vapor diffusion method, X-ray analysis
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.66crystal structures of tetrameric CEAS-thiamin diphosphate in complex with the substrate analogues 5-guanidinovaleric acid and tartrate, crystals of 2 morphologies are grown by the hanging-drop vapour diffusion technique using 1.6 M ammonium sulfate as precipitant, in the presence of 0.1 M HEPES, pH 7.4, and a 3fold molar excess of thiamin diphosphate
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