2.5.1.56 | purified and concentrated enzyme crystallized at 18°C, hanging-drop vapor diffusion, 10 mM MnCl2, 1.50-1.55 M malic acid, pH 6.2, soaked in 2 M sodium phosphate, pH 6.2, with 10 mM MnCl2, 25% ethylene glycol, frozen in liquid nitrogen at -173°C, complex with Mn2+ and 4.5/1 mixture of 2S-inhibitor/2R-inhibitor |
707514 |