   2.4.1.91 | purified recombinant detagged enzyme bound to UDP-2-fluoroglucose and kaempferol, or with UDP and quercetin, hanging drop method, 4.8 mg/ml protein in 5 mM Tris (2-carboxyethyl) phosphine HCl, 10 mM TrisยCl, pH 8.3, diluted 1:1 with mother liquor which contains 18ย22% polyethylene glycol 10 000, 0.1M Bis-Tris propane, pH 7.0, and 0ย0.5% v/v Pluronic F-68, crystals appear within 24 h, X-ray diffraction structure determination and analysis at 2.2 A resolution |
673320 |
| 2.4.1.91 | wild-type and selenomethionine-labeled enzyme in complex with UDP and UDP-glucose, hanging drop vapour diffusion method, 5 mg/ml protein is mixed with 5 mM UDP-galactose and 5 mM quercetin at a 2:1 v/v ratio, mixed with an equal volume of reservoir solution containing 40% w/v PEG 3350, 0.2 M ammonium acetate, and 0.1 M sodium citrate, pH 5.6, equilibration over the reservoir solution at 20°C, 2-5 days, X-ray diffraction structure determination and analysis at 2.0-2.6 A resolution, molecular docking |
676413 |
