2.4.1.7 | recombinant enzyme, hanging drop vapour diffusion method, 0.0025 ml protein solution, containing 0.5-1.0 mg/ml protein, 10 mM Tris-HCl, pH 7.1, is mixed with eual volume of precipitant solution containing 27% w/v PEG 4000, 0.1 M Tris-HCl, pH 8.5, and 0.1 sodium acetate, at 25°C, 3-14 days, X-ray diffraction structure determination and analysis at 1.77 A resolution |
658024 |