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EC Number	Crystallization (Commentary)
2.4.1.232	purified recombinant wild-type and mutant enzymes, sitting drop vapour diffusion, mixing of 0.0005 ml of protein solution with 0.0005 ml of preciputation solution containing 0.1 M HEPES, pH 7.5, and 2 M ammonium sulfate, crystals are soaked in 50% Na-malonate, pH 7.5, containing mersalyl acid for 16 h at 20°C, crystals of ScMnn9-D236N mutant are transferred to 50% Na-malonate, pH 7.5, and soaked with 3 mM GDP and 10 mM MnCl2 for 10 min at 20°C, X-ray diffraction structure determination and analysis at 2.0-2.2 A resolution, modeling

EC Number

Crystallization (Commentary)

2.4.1.232

purified recombinant wild-type and mutant enzymes, sitting drop vapour diffusion, mixing of 0.0005 ml of protein solution with 0.0005 ml of preciputation solution containing 0.1 M HEPES, pH 7.5, and 2 M ammonium sulfate, crystals are soaked in 50% Na-malonate, pH 7.5, containing mersalyl acid for 16 h at 20°C, crystals of ScMnn9-D236N mutant are transferred to 50% Na-malonate, pH 7.5, and soaked with 3 mM GDP and 10 mM MnCl2 for 10 min at 20°C, X-ray diffraction structure determination and analysis at 2.0-2.2 A resolution, modeling

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Release 2023.1 (January 2023)