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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.140purified recombinant truncated mutant ASRDELTA2 (ASR (Met1-Ala2057)), sitting drop vapour diffusion method, mixing of 200 nl of 8 mg/ml protein in 30 mM MES buffer, pH 6.5, 100 mM NaCl, 0.05 g/l CaCl2, and 10 mM isomaltohexaose, with 200 nl of reservoir solution containing 17% w/v PEG 3350, and NaNO3 0.5 M, 3 months, at 12°C, X-ray diffraction structure determination and analysis at 2.8 A resolution
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