2.4.1.13 | purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 15mg/ml protein solution with 0.001 ml of reservoir solution containing 5% Tacsimate, pH 5.0, 5% w/v PEG 3350, and 0.1 M sodium citrate, pH 5.6, 20°C, 7 days, X-ray diffraction structure determination and analysis at 3.05 A resolution, molecular replacement with Arabidopsis thaliana sucrose synthase structural model, PDB ID 3S29, as a template |
736366 |