2.3.2.3 | purified recombinant enzyme in complex with product LysW-gamma-AAA, hanging drop vapor diffusion method, method screening and subsequent optimization, mixing of 7.5 mg/ml enzyme, 2.5 mg/ml TkLysW, 10 mM AMP-PNP, 10 mM alpha-aminoadipate (AAA), or 50 mM glutamate, and 10 mM MgSO4 with crystallization solution containing 20% v/v 2-methyl-2,4-pentanediol, 6.6% w/v PEG 3350, 0.066 M imidazole, pH 6.5, and 0.132 M ammonium sulfate, 20°C, X-ray diffraction stucture determination and analysis at 2.18 A resolution |