EC Number   |
Reference |
|---|
   2.3.1.269 | purified recombinant C-terminally His8-tagged enzyme, 15 mg/ml protein in 25 mM Tris, pH 8.0, 100 mM NaCl, 5% glycerol, 0.5 mM TCEP, and 0.02% DDM, is mixed with 25% PEG 2000 MME, 100 mM Na cacodylate, pH 6.4, and 40 mM MgCl2 with a drop ratio of 1:1.75 protein:precipitate, 18°C, 2 weeks. For the N-terminally cleaved detagged enzyme, 36% PEG 200, 400 mM ammonium phosphate dibasic, and 100 mM HEPES, pH 7.2, at 10°C for 3 weeks, are used. X-ray diffraction structure determination and analysis at 3.1-3.5 A resolution, modeling |
758353 |
| 2.3.1.269 | purified recombinant wild-type and selenomethionine-labeled enzymes, hanging drop vapor diffusion method, 12 mg/ml protein from 50 mM Tris-HCl, pH 7.5, and 26% v/v PEG 550 MME supplemented with two detergents, n-heptyl-beta-D-thioglucopyranoside and CHAPSO, 16°C, X-ray diffraction structure determination and analysis at 2.59 and 3.60 A resolution, respectively |
757766 |
| 2.3.1.269 | using 30% (w/v) PEG-500 DME, 0.1 M sodium citrate pH 5.0 and 0.1 M sodium acetate |
748746 |
| 2.3.1.269 | using 8% (w/v) 2-methyl-2,4-pentanediol, 0.1 M MES pH 6.0 and 0.4 M ammonium citrate |
748746 |
| 2.3.1.269 | vapor diffusion method, using 50 mM ADA, pH 6.5, and 24% (w/v) PEG 400 |
749188 |
