EC Number   |
Reference |
|---|
    2.2.1.7 | enzyme bound to C2alpha-phosphonolactyl-thiamine diphosphate and a native post-decarboxylation state with a bound enamine intermediate |
757220 |
| 2.2.1.7 | purified recombinant wild-type and selenomethionine-labeled enzyme in complex with cofactor thiamine diphosphate, mixing of protein solution with reservoir solution containing 150 mM NaCl, and 20% w/v PEG 6000 or PEG 8000, cryoprotection by 25% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 2.9 A resolution |
674856 |
| 2.2.1.7 | purified recombinant wild-type and selenomethionine-labeled enzyme in complex with cofactor thiamine diphosphate, protein solution is mixed with the reservoir solution containing 30% w/v PEG 3350 and 200 mM Na,K-tartrate and infected by a fungus, the enzyme crystallized only after in situ proteolysis by a fungal protease, cryoprotection by 25% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 2.4 A resolution |
674856 |
