EC Number   |
|---|
   2.1.1.57 | by vapour-diffusion, to 2.7 A resolution, in complex with S-adenosyl-l-methionine, crystals belong to the orthorhombic space group P21212, with unit-cell parameters a = 58.7, b = 126.6, c = 156.1 A, presence of three MTase molecules in the asymmetric unit, with a solvent content of 57.7%, residues Lys62, Asp147, Lys184 and Glu220 specifically characterize the active-site region |
| 2.1.1.57 | by vapour-diffusion, to 2.8 A resolution, in complex with S-adenosyl-l-methionine, crystals belong to the tetragonal space group P43, with unit-cell parameters a = b = 83.0, c = 170.2 A, presence of four MTase molecules per asymmetric unit and a corresponding solvent content of 49.9%, residues Lys62, Asp147, Lys184 and Glu220 specifically characterize the active-site region |
| 2.1.1.57 | hanging drop method at room temperature, 0.1 M sodium citrate pH 5.8, 1.2 M lithium sulfate, 0.5 M ammonium sulfate, vapour diffusion for 1 week, structure analysis with and without bound nucleotide, model |
| 2.1.1.57 | hanging drop vapor diffusion method, using in 0.1 M MES, pH 5.0, 2 M NaCl, 0.1 M NaH2PO4, 0.1 M KH2PO4 after 24 h at 25°C |
| 2.1.1.57 | in complex with ribavirin 5-triphosphate and S-adenosyl-L-methionine |
| 2.1.1.57 | to 2.5 A resolution. Residues 175 to 377 encode a distinct 2'-O MT domain segregated from the other domains and responsible for methylation of cap0 structure |
