EC Number   |
|---|
    1.7.2.6 | - |
| 1.7.2.6 | hanging drop vapor diffusion method, using 2.4 M ammonium sulfate, 50 mM Tris-HCL, at pH 8.0 |
| 1.7.2.6 | microbatch-under-oil method, using 0.1 M potassium nitrate, 0.1 M MES pH 7.5 and 46% (v/v) PEG 400 |
| 1.7.2.6 | purified enzyme, 2 weeks |
| 1.7.2.6 | to 2.1 A resolution. Heme P460 contains two covalent cross-links between the porphyrin and a Tyr residue. When purified from source, an unknown physiological HAO binding partner NE1300 is present within the crystal. Protein NE1300 may play a structural role in the ternary complex with cytochrome c554, the physiological electron acceptor of the enzyme |
| 1.7.2.6 | using 0.1 M potassium nitrate, 0.1 M MES pH 7.5 and 46% (w/v) PEG 400 |
