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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.6.99.1-
Display the word mapDisplay the reaction diagram Show all sequences 1.6.99.1purified enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of protein solution containing 14 mg/mL in 25 mM Tris-HCl, pH 8.0, containing 100 mM NaCl, and 1 mM 2-mercaptoethanol, with 0002 ml of 28% w/v PEG 1500, and 0.3 M ammonium fluoride, equilibration against 0.5 ml of reservoir solution, 19°C, 10 days, crystalline forms I and II, X-ray diffraction structure determination and analysis at 1.27 A and 2.0 A resolution, respectively
Display the word mapDisplay the reaction diagram Show all sequences 1.6.99.1purified recombinant enzyme in a ternary complex with inhibitor 12-oxophytodienoate and FMN, X-ray diffraction structure determination and analysis at 2.05 A resolution
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