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EC Number
Crystallization (Commentary)
1.5.1.30
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1.5.1.30
in NAD(P)(+)-free, NAD(+)-bound, and NADP(+)-bound states. The nicotinamide of NAD+ stacks the isoalloxazine ring of FMN so that NADH can directly transfer hydride. The bound NADP+ also has a compact conformation but is bound in a reverse direction, which is not suitable for hydride transfer
1.5.1.30
selenomethionine derivative of mutant L114M/L165M
1.5.1.30
sitting drop vapour diffusion method, using 0.2 M calcium chloride and 20% (w/v) polyethylene glycol 3350
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