1.3.1.48 | crystal structure of the bifunctional leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase, the binary complex structure with NADP+, and the ternary complex structure with NADP+ and 15-oxo-prostaglandin E2, batch method using the automated crystallization system TERA by mixing equal volumes of protein solution (20 mM Tris-HCL, pH 8.0, 150 mM NaCl, 1 mM dithiothreitol) and precipitating solution (100 mM 4-morpholineethanesulfonic acid, pH 5.5-6.8), 17.5-27.5% polyethylene glycol 4000, 50 mM MgCl2 at 18°C |