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EC Number Crystallization (Commentary) Reference
Show all pathways known for 1.3.1.32Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.32purified recombinant His-tagged wild-type and SeMet-labeled PnpD, the sitting-drop vapour-diffusion method, 0.002 ml of 15 mg/ml protein in 20 mM Tris, pH 8.0, 5 mM NADH, and 20 mM NaCl are mixed with 0.002 ml of precipitant solution containing 0.2 M ammonium phosphate dibasic and 20% w/v PEG 3350, equilibration against 0.2 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 2.9 A resolution 710729
Show all pathways known for 1.3.1.32Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.32sitting drop vapor diffusion method, using 1.1-1.4 M ammonium sulfate, 0.1 M sodium chloride, 2% (w/v) benzamidine-HCl and 0.1 M Na HEPES pH 7.5 at 20°C 741383
Show all pathways known for 1.3.1.32Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.32sitting drop vapour diffusion method, using ammonium sulfate as the precipitating agent, at 20°C 684201
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