EC Number   |
|---|
   1.3.1.20 | - |
| 1.3.1.20 | complexed with NADP+ and testosterone, 10fold molar excess of NADP+, 'waterbug' dialysis method in 10 mM potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM 2-mrcapthoethanol, 5% acetonitrile and 0.2 mM testosterone, and crystallization in presence of polethylene glycol 8000 in hanging drops at room temperature, single crystals |
| 1.3.1.20 | cryocrystallization: crystals were soaked in 21% polyethylene glycol 8000, 0.1 M sodium cacodylate, pH 6.4, 1.25 mM NADP+, 0.1 mM testosterone and 15% 2-methyl-2,4-pentanediol as cryoprotectant |
| 1.3.1.20 | crystals are obtained at 19.9°C in a culture plate via the vapour-diffusion method, crystal structure of dimeric dihydrodiol dehydrogenase complexed with the inhibitor isoascorbic acid is determined at 2.59 A resolution |
| 1.3.1.20 | dimeric apoenzyme (at 2.0 A resolution) and enzyme-inhibitor complex (at 2.9 A resolution), crystals are grown using droplets consisting of the dihydrodiol dehydrogenase/NADPH solution mixed with a matching volume containing 1.5 M ammonium phosphate and 0.1 M sodium citrate at pH 5.6, tertiary structure is formed by a classical dinucleotide binding fold comprising of 2 beta-alpha-beta-alpha-beta motifs at the N-terminus and an eight-stranded, predominantly antiparallel beta-sheet at the C-terminus |
| 1.3.1.20 | in complex with isoascorbic acid, vapour diffusion method with 2 M ammonium sulfate, 0.1 M sodium citrate buffer pH 5.0, at 20°C |
| 1.3.1.20 | structure determination, cofactor and substrate binding site, and model building |
| 1.3.1.20 | vapour diffusion at 22°C in 100 mM sodium citrate, polyethylene glycol 6000 25%, 120 mM ammonium sulfate, pH 5.8, x-ray analysis |
| 1.3.1.20 | vapour diffusion at 295 K with NADPH, growth for 2 days, buffer A: 1.5 M ammoinum phosphate, 0.1 M sodium citrate, pH 5.6 + buffer B: 10 mM Tris-HCl, 2 mM 2-mercaptoethanol, pH 8.5, preliminary x-ray structure analysis |
| 1.3.1.20 | vapour diffusion method with 2 M ammonium phosphate and 0.1 M sodium citrate buffer (pH 5.6) and 20% glycerol |
