EC Number   |
Reference |
|---|
   1.2.4.4 | - |
349040, 349088, 687543 |
| 1.2.4.4 | 20-25 mg/ml wild-type or recombinant His-tagged E1b component in 50 mM HEPES, pH 7.5, 0.25 M KCl, 0.5 mM PMSF, 1 mM benzamidine, 20 mM DTT, 5% v/v glycerol, vapour diffusion method, 20°C, mixing with equal volume of well solution containing 1.4-1.6 M ammonium sulfate, 0.1 M sodium citrate, pH 5.8, 20 mM 2-mercaptoethanol, 4 mM MgCl2 or MnCl2, 4 mM thiamine diphosphate, maximal size after 10 day after microseeding, cryoprotection by well solution with 20% v/v glycerol, X-ray diffraction structure determination and analysis at 1.85-2.25 A resolution |
656171 |
| 1.2.4.4 | crystallization of 4 forms of complex component E1: 1. E1 apoenzyme, 2. E1 holoenzyme, 3. E1 holoenzyme in complex with substrate analogue 4-methylpentanoate, 4. E1 holoenzyme in complex with substrate 4-methyl-2-oxopentanoate, hanging drop vapour diffusion method, 18°C, 0.002 ml 10 mg/ml purified recombinant protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM DTT, with equal volume of 0.002 ml of reservoir solution containing 0.7 M lithium sulfate, 60 mM sodium citrate, pH 5.6, against 0.4 ml reservoir solution, a few days, X-ray diffraction structure determination and analysis at 1.9-2.4 A resolution |
656534 |
| 1.2.4.4 | crystals of E1b grown in the absence and presence of substrates at 22ºC via the vapor-diffusion method, key tyrosine residue in the E1b active site, functions as a conformational switch to reduce the reactivity of the thiamin diphosphate cofactor, the tyrosine switch further remodels an E1b loop region to promote E1b binding to E2b |
677159 |
| 1.2.4.4 | purified wild-type and C-terminally His-tagged recombinant E1b component and mutants, 22°C, hanging drop vapour diffusion method, Mg2+ or Mn2+, X-ray diffraction structure determination and analysis at 1.8 A resolution |
656230 |
