EC Number |
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1.2.4.2 | enzyme in complex with phosphono ethyl succinyl phosphonate and/or succinyl phosphonate, hanging drop vapor diffusion method, using 2% (w/v) 2-methyl-2-propanol and 47% (w/v) 2-methyl-2,4-pentanediol |
1.2.4.2 | in complex with inhibitors succinyl phosphonate, phosphono ethyl succinyl phosphonate. Crystallographic snapshots of the Michaelis-like complex, followed by the covalent adduction of the inhibitor to thiamin diphosphate, mimicking the pre-decarboxylation complex |
1.2.4.2 | sitting drop vapour diffusion method with 12% (w/v) polyethylene glycol 4000, 50 mM sodium citrate (pH 5.6) |