EC Number   |
|---|
    1.2.1.59 | - |
| 1.2.1.59 | crystal structure is determined to 1.81 A resolution. The crystal belongs to space group C222(1), with unit-cell parameters a = 83.4, b = 152.0, c = 118.6 A. Crystallization is performed using the microbatch-under-oil method at 18°C |
| 1.2.1.59 | purified enzyme in complex with ADP, NAD+, and NADP+, X-ray diffraction structure determination and analysis at 2.05-2.25 A resolution |
| 1.2.1.59 | purified His-tagged enzyme in complex with NAD+, microseeding method, mixing of 8.9 mg/ml protein in 25 mM HEPES, pH 7.35, 0.1 M NaCl, and 5 mM 2-mercaptoethanol, with crystallization solution containing 26-36% PEG 4000, 0.1 M MES, pH 6.5, the needle-like crystals are used for microseeding by mixing 0.002 ml of protein plus cofactor mixture, 500 nl water and 500 nl reservoir solution containing seed suspension in 28% PEG 4000, 0.1 M MES, pH 6.5, X-ray diffraction structure determination and analysis at 2.46 A resolution, molecular replacement and modelling |
| 1.2.1.59 | wild type and mutant enzyme C150S, sitting drop vapor diffusion method, using 25% (w/v) PEG 1K, 175 mM sodium/potassium tartrate and 100 mM sodium acetate pH 4.5 |
