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EC Number	Crystallization (Commentary)
1.16.1.10	crystals of the homodimeric enzyme are grown at 4°C by the hanging drop vapor diffusion technique, with protein solution at a concentration of 25 mg/ml in 0.1 M Tris-HCl (pH 7.5) and a reservoir consisting of 21%22% w/v PEG4000, 0.1 M sodium acetate, and 0.1 M Tris-HCl (pH 8.4). The crystallization drops are microseeded after 2024 hr. Crystal structure of recombinant enzyme containing a bound FMN solved at 1.5 A resolution. The NADP+-bound enzyme complex is determined at 1.65 A resolution

EC Number

Crystallization (Commentary)

1.16.1.10

crystals of the homodimeric enzyme are grown at 4°C by the hanging drop vapor diffusion technique, with protein solution at a concentration of 25 mg/ml in 0.1 M Tris-HCl (pH 7.5) and a reservoir consisting of 21%22% w/v PEG4000, 0.1 M sodium acetate, and 0.1 M Tris-HCl (pH 8.4). The crystallization drops are microseeded after 2024 hr. Crystal structure of recombinant enzyme containing a bound FMN solved at 1.5 A resolution. The NADP+-bound enzyme complex is determined at 1.65 A resolution

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Release 2023.1 (January 2023)