EC Number   |
|---|
    1.16.1.1 | hanging-drop vapor-diffusion method |
| 1.16.1.1 | purified recombinanat His-tagged enzyme Tn501 MerA, as NADP+/Hg2+ complex of mutant C136A/C141A, hanging drop vapor diffusion technique, mixing of 0.002 ml of 25 mg/ml protein solution with 0.0.02 ml of reservoir solution containing 0.1 M Tris, pH 9.4, 20 mM NADP+, 2.0 M (NH4)2SO4, equilibration against 0.5 ml of reservoir solution, X-ray diffraction structure determination and analysis at 1.6 A resolution, modeling |
| 1.16.1.1 | purified recombinanat His-tagged enzyme, vapour diffusion hanging drop method, from 0.085 M Tris, pH 8.5, 15% v/v glycerol, 14% w/v PEG 400, 0.19 M LiSO4, and 20 mg/ml protein, 2 weeks, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular replacement using Tn501 MerA, PDB ID 1ZK7, as template, modeling |
| 1.16.1.1 | purified recombinant His-tagged enzyme, mixing of 10 mg/ml protein in 50 mM phosphate buffer, pH 7.2, with reservoir solution containing 5% v/v Tascimate, 0.1 M HEPES, pH 7.0, and 10% w/v PEG monomethyl ether 5000, X-ray diffraction structure determination and analysis at 3.48 A resolution |
