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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.B28molecular modeling of structure
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.B28vapor diffusion method using a protein solution (15 mg/ml) and a reservoir solution (30% (w/v) polyethylene glycol 4000, 200 mM lithium sulfate monohydrate and 100 mM Tris-HCl, pH 8.5)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.B28vapour diffusion method, X-ray crystallography at a resolution of 1.94 A reveals a sufficiently large heme pocket for NAD(P)H binding and a novel contiguous channel from the active site to bulk solvent in the distal heme pocket. The mutant shows a higher affinity for NADH compared with the wild-type because the mutant has a more widely open distal pocket for NAD(P)H binding
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