1.14.13.149 | hanging drop vapor diffusion method, in the absence of a bound ligand (with 100 mM N-(2-acetamido)-iminodiacetic acid pH 5.5) as well as in complexes with CoA, 3-hydroxybutyryl-CoA, benzoyl-CoA and phenylacetyl-CoA, using either 0.1 M PIPES pH 6.5, 15% (w/v) PEG 550 monomethyl ether or 0.1 M PIPES pH 6.5, 5% (v/v) 2-propanol, 5% (w/v) PEG 550 monomethyl ether |
717032 |