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EC Number Crystallization (Commentary) Reference
Show all pathways known for 1.14.13.149Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.149hanging drop vapor diffusion method, in the absence of a bound ligand (with 100 mM N-(2-acetamido)-iminodiacetic acid pH 5.5) as well as in complexes with CoA, 3-hydroxybutyryl-CoA, benzoyl-CoA and phenylacetyl-CoA, using either 0.1 M PIPES pH 6.5, 15% (w/v) PEG 550 monomethyl ether or 0.1 M PIPES pH 6.5, 5% (v/v) 2-propanol, 5% (w/v) PEG 550 monomethyl ether 717032
Show all pathways known for 1.14.13.149Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.149hanging drop vapor diffusion method, PaaAC with acetyl-CoA is crystallized in 0.1 M sodium citrate buffer, pH 5.5, and 15% (w/v) PEG 6000 (Fluka). Crystals of ligand-free PaaAC are obtained in 100 mM N-(2-acetamido)-iminodiacetic acid, pH 5.5 717818
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