EC Number   |
|---|
   1.1.3.17 | active site of wild-type choline oxidase, resolution of 1.86 A |
| 1.1.3.17 | anaylsis of biophysical properties of choline oxidase |
| 1.1.3.17 | crystal structure determined by synchrotron X-ray radiation, refined to a resolution of 1.86 A, data collected at 100 K |
| 1.1.3.17 | hanging drop vapor diffusion method, 1.2-1.8 M ammonium sulfate and 10% (v/v) DMSO in 0.1 M Bis-Tris propane (pH 8.5), flash-frozen in liquid nitrogen. Analysis of the flavin C4a-oxygen adduct in choline oxidase |
| 1.1.3.17 | in complex with glycine betaine, hanging drop vapor diffusion method, using 0.1 M magnesium acetate pH 6.0, 50 mM calcium chloride, 2.5% (v/v) glycerol, 10% (w/v) PEG 6000 |
| 1.1.3.17 | mutant enzyme S101A, hanging drop vapor diffusion method, using 80 mM sodium cacodylate, 20% (v/v) PEG6000, 20% (v/v) glycerol, 150 mM Mg-acetate at pH 6.0 |
| 1.1.3.17 | purified recombinnat wild-type enzyme and mutant V464A, hanging drop vapor diffusion, room temperature, from 10-15% v/v PEG 6000, 50-200 mM magnesium acetate, 200 mM trimethylamine, and 0.08 M sodium cacodylate, pH 6.0, X-ray diffraction structure determination and analysis at 2.2 A resolution |
