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Results 1 - 4 of 4
EC Number Crystallization (Commentary) Reference
Show all pathways known for 1.1.1.81Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.81in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl 739674
Show all pathways known for 1.1.1.81Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.81purified detagged recombinant enzyme in ternary complex with product D-glycerate and cofactor NADPH, sitting drop vapour diffusion method, 5.5 mg/ml protein in 20 mM Tris-HCl, pH 8.5, 1 mM 2-mercaptoethanol, 0.2 mM NADPH, and 0.5 mm di-sodium oxalate, mixed with mother liquor, containing 15% w/v PEG 8000, 0.2 M ammonium sulfate, and 0.1 M sodium cacodylate, pH 6.5, to 0.002 ml drops, 18°C, X-ray diffraction structure determination and analysis at 2.2 A resolution 669886
Show all pathways known for 1.1.1.81Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.81sitting drop vapor diffusion method in the presence of NAD, crystal structure analysis reveals tightly bound NADP(H) at the enzyme originating from Escherichia coli expression, which is not replaceable by NAD 684141
Show all pathways known for 1.1.1.81Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.81structures in apo forms and in complexes with cofactors and substrates/substrate analogues 760598
Results 1 - 4 of 4