1.1.1.60 | enzyme with L-tartrate bound to the active site, sitting drop vapour diffusion method, mixing of 0.0005 ml of the protein solution, containing 107 mg/ml protein in in 10 mM HEPES pH 8.0, 200 mM NaCl, with 0.0005 ml of 0.1 M imidazole buffer pH 8.0, 1 M potassium/sodium L-tartrate and 0.2 M sodium chloride, and equilibrated at 16°C over 0.135 ml of this solution, overnight, X-ray diffraction structure determination and analysis at 1.65 A resolution, the single-wavelength anomalous diffraction method |