   1.1.1.378 | purified recombinant enzyme in ligand-free, NAD+-bound, NADP+-bound, and L-rhamnose- and NAD+-bound forms, sitting drop vapor diffusion method, mixing of 500 nl of 10 mg/ml protein in 20 mM Tris/HCl, pH 8.0, and 150 mM NaCl, with 500 nl of reservoir olution containing 100 mM Tris/HCl, pH 8.5, 200 mM Li2SO4, and 25% w/v PEG 3350, with or without coenzyme, for the apoenzyme and for the NADP+-bound form,, and 100 mM Tris/HCl, pH 8.0, 200 mM NaCl, 24.5% w/v PEG 3350, and 3% v/v glycerol only for the NAD+-/L-rhamnose-bound form, equilibration against 0.07 ml of reservoir solution, at 20°C, X-ray diffraction structure determination and analysis at 1.9, 2.1, 2.4, and 1.6 A resolution, respectively, molecular replacement using the structure of 3-oxoacyl-(acyl carrier protein) reductase from Bacillus anthracis (PDB ID 2UVD) as the search model, modeling |
