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Results 1 - 5 of 5
EC Number Crystallization (Commentary)
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.363cocrystallization of and mutant enzyme Q365, mutant enzymen S215, mutant enzyme S215 with NAD+ and mutant enzyme Q365 with NADP+, hanging-drop vapour diffusion method, structures of NADP+- and NAD+-complexed enzymes are determined at 2.2 and 2.5 A resolution
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.363crystallized from phosphate buffer in a form suitable for X-ray crystallographic studies. The crystals diffract to better than 2.4 A. The space group is P3(1)21 (P3(2)21), a = 105.8 A, c = 225.1 A, V = 2.18 X 10(6) A(3). The asymmetric unit probably contains a single dimer
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.363hanging drop method, crystallization of six cysteine-containing mutants (Q56C, S61C, S132C, S215C, Q365C, S428C) of the enzyme and the successful preparation and crystallization of a heavy atom derivative of mutant enzyme S215C
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.363hanging drop vapor diffusion technique, determination of the three-dimensional structure of the D177N mutant enzyme by X-ray cryocrystallography in the presence of NAD+ and in the presence of glucose 6-phosphate plus NADPH. The structure of a glucose 6-phosphate complex of a mutant (Q365C) with normal enzyme activity is also determined and substrate binding compared
Show all pathways known for 1.1.1.363Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.363the three-dimensional structure of the H240N glucose 6-phosphate dehydrogenase is determined at 2.5 A resolution. Crystals are grown in the presence of either glucose 6-phosphate and NAD+ or glucose 6-phosphate and NADP+, hanging drop vapor diffusion method with 2.27 M ammonium sulfate
Results 1 - 5 of 5