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Results 1 - 6 of 6
EC Number Crystallization (Commentary) Reference
Show all pathways known for 1.1.1.36Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.365 mg/ml purified recombinant, detagged truncated enzyme, amino acid residues 1-604, by vapor diffusion technique, 20°C, in 30% PEG 4000, 0.1 M sodium acetate, pH 4.2, 0.2 M ammonium acetate, equal volume of 0.002 ml of protein and precipitant solution, 11% v/v glycerol as cryoprotectant, X-ray diffraction structure determination and analysis at 2.22-2.36 A resolution, the wild-type enzyme, comprising amino acid residues 1-906, is unstable and not crystallizable 654460
Show all pathways known for 1.1.1.36Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.36apo and NADP+-bound enzyme forms, sitting drop vapor diffusion method, using 100 mM Na-HEPES (pH 7.5), 30% (v/v) PEG 400 and 200 mM magnesium chloride hexahydrate for the apo enzyme and 200 mM sodium citrate tribasic dehydrate and 20% (w/v) PEG 3350 for the NADP+-bound enzyme 760524
Show all pathways known for 1.1.1.36Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.36hanging-drop vapour diffusion 669878
Show all pathways known for 1.1.1.36Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.36purified recombinant His-tagged wild-type and mutant enzymes in complex with NADP+ and acetoacetyl-CoA, wild-type protein crystals grow from 0.1 M MES, pH 7.1, 0.9 mM NADP+, 0.9 mM acetoacetyl-CoA, 1.6 M ammonium sulfate, and 10% 1,4-dioxane, mutant crystals grow from 0.6-1.2 M sodium-potassium tartrate, 0.16-0.20 M lithium sulfate, and 0.1 M CHES, pH 8.9-9.9, X-ray diffraction structure determination and analysis at 1.8 A and 2.0-2.9 A resolution, respectively, molecular replacement method using the structure of FabG from Escherichia coli, PDB ID 1I01, as the search probe 739901
Show all pathways known for 1.1.1.36Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.36selenomethionine-labeled recombinant truncated enzyme, X-ray diffraction structure determination and analysis at 2.38 A resolution 657366
Show all pathways known for 1.1.1.36Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.36sitting drop vapor diffusion method, using 10% (w/v) PEG 8000, 8% (w/v) ethylene glycol, 100 mM HEPES-NaOH pH 7.5 760305
Results 1 - 6 of 6