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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.201enzyme bound to NADP+, sitting drop vapor diffusion method, using 0.1 M sodium malonate pH 7.0, 12% (w/v) polyethylene glycol 3350
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.201purified His-tagged enzyme complexed with NADP+, mixing of 10-15 mg/ml protein in 20% v/v glycerol, 20 mM potassium phosphate, pH 7.0, and 5 mM NADP+, with1 M sodium formate, 100 mM sodium acetate, pH 4.6, at room temperature, 3-4days, X-ray diffraction structure determination and analysis at 1.6 A resolution, modelling, molecular replacement using the the apoform structure of 7alpha-HSDH from Escherichia coli as search model, PDB ID 1AHH
Results 1 - 2 of 2