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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.159-
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.159binary complex with NAD+, ternary complex with NADH and 7-oxoglycochenodeoxycholic acid as product, as well as possibly partially glycochenodeoxycholic acid as substrat
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.159purified recombinant enzyme complexed with taurochenodeoxycholic acid and NADP+, sitting drop vapour diffusion method, mixing of 200 nl of 1.12 mM protein in 50 mM Tris-HCl, pH 8.0, 200 mM NaCl,and 5.6 mM of NADP+ and taurochenodeoxycholic acid, with 200 nl of reservoir solution containing 0.1 M HEPES, pH 7.5, and 25% PEG-3350, and equilibration against 0.03 ml reservoir solution, at 20°C, X-ray diffraction structure determination and analysis at 2.0 A resolution
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