1.1.1.157 | apo and NAD+-bound enzyme forms, hanging drop vapor diffusion method. NAD+-bound crystals grow using a reservoir solution consisting of 17.5% (w/v) PEG 3350, 200 mM sodium thiocyanate. Apo crystals grow using a reservoir solution consisting of 1.25% (w/v) PEG 400, 2.2 M ammonium sulfate, 0.1 M HEPES-NaOH pH 7.5 |