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reaction involves the intramolecular transfer of ammonia between two spatially separated active sites, molecular dynamics simulations on wild-type and mutant structures to investigate the transport of ammonia along two putative tunnels
the glutamine transamidosome complex consisting of tRNAGln, glutamyltRNA synthase (GluRS) and the heterotrimeric amidotransferase GatCAB is crystalyzed at 3.35 A. Crystals are grown by the sitting-drop vapour-diffusion method at 20°C
to 3.3 A resolution
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