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Crystallization (Commentary)
purified recombinant N-terminal domain of ScGS, unliganded and in complex with 3 Mg2+ ions and alendronate, mixing of 600 nl of 7 mg/ml protein in 25 mM Tris, pH 8.2, 5 mM MgCl2, 10 mM BME, and 1.5 mM sodium alendronate, with 600 nl of precipitant solution containing 0.2 M sodium acetate trihydrate, pH 7.0, and 20% w/v PEG 3350, and equilibration against 0.1 ml of reservoir solution at room temperature, X-ray diffraction structure determination and analysis at 2.4 A resolution. Neither full-length ScGS nor constructs of the C-terminal domain can be crystallized, but homology models of the C-terminal domain are constructed based on about 36% sequence identity with the N-terminal domain
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