EC Number   |
Reference |
|---|
   4.1.2.52 | crystallization of apo-HpcH, because divalent metal ion is lost during the purification process, resulting in preparation of the inactive apo form of the enzyme, crystals grown using the hanging-drop vapour diffusion method |
722955 |
| 4.1.2.52 | HpcHMg2+oxamate crystals grown with the addition of 10 mM magnesium chloride and sodium oxamate (substrate analogue), elucidation of active site architecture |
722955 |
| 4.1.2.52 | in complex with substrate or products, hanging drop vapor diffusion method, using |
725493 |
| 4.1.2.52 | the active site of HpaI is formed by approx. 30 residues from adjacent dimers and consists of an approx. 15 A deep bell-shaped cleft with an approx. 12 A wide mouth. This broad entrance to the active site is predominantly lined with noncharged residues and a few positively charged residues |
702377 |
