3.1.3.97 | unliganded enzyme, sitting drop vapor diffusion method, using 25% (w/v) PEG3350, 200 mM (NH4)2SO4, and 100 mM Bis-Tris (pH 6.5). Enzyme with AMP and phosphate bound in the active site, sitting drop vapor diffusion method, using 20% (w/v) PEG 2000 MME and 100 mM Bis-Tris (pH 6.0) |