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Results 1 - 6 of 6
EC Number
Crystallization
Reference
crystal structures of the S117A and Y44F mutants of cocE
hanging drop vapor diffusion method, using 0.1 M phosphate-citrate (pH 4.2), 1.6 M sodium dihydrogen phosphate, and 0.4 M dipotassium hydrogen phosphate
hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 100 mM 2-(N-morpholino)-ethane sulfonic acid, pH 6.0, and 1 M NaCl
mutant enzyme L169K/G173Q, hanging drop vapor diffusion method, using 1.7 M ammonium sulfate, 10 mM Tris-HCl, pH 7.3, and 25 mM NaCl
te crystal structure of cocE, solved by multiple anomalous dispersion methods, reveals that cocE is a serine esterase composed of three domains: (1.) a canonical alpha/beta hydrolase fold (2.) an alpha-helical domain that caps the active site and (3.) a jelly-roll-like beta-domain that interacts extensively with the other two domains. The active site is identified within the interface of all three domains by analysis of the crystal structures of transition state analog adduct and product complexes, which are refined at 1.58 A and 1.63 A resolution, respectively
Results 1 - 6 of 6