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EC Number Crystallization (Commentary) Reference
Show all pathways known for 2.9.1.1Display the word mapDisplay the reaction diagram Show all sequences 2.9.1.1apoenzyme and in complex with Thermoanaerobacter tengcongensis tRNASec, vapor diffusion method 739706
Show all pathways known for 2.9.1.1Display the word mapDisplay the reaction diagram Show all sequences 2.9.1.1purified recombinant C-terminally His6-tagged SecS, apoenzyme or in complex with phosphate or iodide, sitting-drop vapor diffusion at 20°C, 14 mg/ml protein in 10 mM HEPES-NaOH, pH 7.5, 500 mM NaCl, 2 mM DTT, is mixed with an equal volume of reservoir solution containing 11% v/v ethylene glycol without other buffer components, 2 days, cryoprotection by crystal soaking in 100 mM HEPES-NaOH, pH 7.5, 250 mM NaCl, 1 mM dithiothreitol, and 35% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 1.65-2.38 A resolution 693212
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