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EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.7.2.3hanging drop vapor diffusion method 392771, 437735
Display the word mapDisplay the reaction diagram Show all sequences 1.7.2.3recombinant monomeric, dimeric, and trimeric forms, hanging drop vapour diffusion method, 4°C, 0.001 ml of 1.2 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 220 mM NaCl, 10 mM DTT, mixed with equal volume of reservoir solution containing 1.6 M ammonium sulfate, 100 mM MES, pH 6.4, 4-6 days, cryoprotection by addition of 15% w/v ethylene glycerol to the reservoir solution, X-ray diffraction structure determination and analysis at 2.42 A resolution 657290
Results 1 - 2 of 2