EC Number |
Reference |
---|
1.3.98.1 | - |
390915, 390916, 684914 |
1.3.98.1 | apo-enzyme and in complex with orotate and with fumarate, to 2.0 A, 2.5 A and 1.9 A resolution, respectively. Both orotate and fumarate bind to the same active site and exploit similar interactions, consistent with a ping-pong mechanism. Rearrangements in the conformation of the catalytic loop have direct influence on the dimeric interface |
724484 |
1.3.98.1 | by sitting-drop vapor-diffusion technique, to 2.4 A resolution |
671050 |
1.3.98.1 | by the vapour-diffusion technique using lithium sulfate as the precipitating agent, to better than 2.0 A resolution, crystals belong to space group P61, presence of two molecules in the asymmetric unit |
671092 |
1.3.98.1 | crystallized by the vapour-diffusion technique using sitting and hanging drops (lithium sulfate as the precipitating agent). The crystals belong to space group P6(1), with unit-cell parameters a = 143.7, c = 69.8 A. X-ray diffraction data are collected to 2.0 A resolution using an in-house rotating-anode generator |
671092 |
1.3.98.1 | crystals of the TcDHODorotate complex are grown at 4°C by the sitting-drop vapour-diffusion technique using polyethylene glycol 3350 as a precipitant. The crystals diffract to better than 1.8 A ° resolution using synchrotron radiation (lambda = 0.900 A). X-ray diffraction data are collected at -173°C and processed to 1.9 A ° resolution with 98.2% completeness. The TcDHOD crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.87, b = 71.89, c = 123.27 A |
671086 |
1.3.98.1 | DHODorotate complex by the sitting-drop vapour-diffusion technique using polyethylene glycol 3350 as a precipitant, to better than 1.8 A resolution, crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 67.87, b = 71.89, c = 123.27 A |
671086 |
1.3.98.1 | dihydroorotate dehydrogenase A, complexed with orotate |
390915 |
1.3.98.1 | enzyme in complex with orotate, crystal structure analysis, overview |
711915 |
1.3.98.1 | hanging drop vapor diffusion technique, 30% polyethylene glycol, 0.2 M sodium acetate, 0.1 M Tris-HCl, pH 8.5, monoclinic crystals, space group P21 |
390916 |